Greek Scientists Find Cell Response Mechanism Promising Drug Targets for Blood Cancer

Dr. Iannis Aifantis

Greek scientists at New York University School of Medicine led a research team that has found promising drug targets against blood cancer, a medicalxpress.com report says.

Dr. Iannis Aifantis and Dr. Nikos Kourtis lead the team that found a signaling pathway that helps promote normal cell growth which impacts a form of leukemia by taking control of another pathway better known for protecting cells from biological stress.

The discovery that the NOTCH1 pathway takes control of heat shock transcription factor 1 (HSF1) signaling in T cell acute lymphoblastic leukemia, or T-ALL, suggests that blocking one or more genes in the HSF1 pathway could represent a new approach in treating the aggressive disease, the researchers say.

According to the medicalxpress.com report, researchers say their study is the first to directly link activation of HSF1, which is critical to the production of dozens of other proteins, including HSP90, to any leukemia.

“Our study shows how the NOTCH1 pathway hijacks the heat shock transcription factor 1 pathway to promote tumor growth,” says study senior investigator Dr. Iannis Aifantis, Ph.D., professor and chair of the Department of Pathology at NYU Langone Health and its Perlmutter Cancer Center. “The cancer cells are sending into overdrive a system that helps healthy cells respond to stress.”

A drug blocking HSP90 is at a trial state. Dr. Aifantis says if further tests prove successful, the experimental drug, labeled PU-H71, could be quickly adapted for trials in T-ALL patients.

Researchers found through tests of the drug in animals and human cells show that blocking HSP90 kills only cancer cells. Therefore, Dr. Aifantis says its use is likely to have fewer side effects than current T-ALL treatments, such as chemotherapy, which kills both normal and cancer cells.

“Having a targeted therapy that kills only cancer cells could really boost our efforts to treat T cell acute lymphoblastic leukemia, which affects mostly children,” says study first author Nikos Kourtis, Ph.D., a postdoctoral fellow at NYU Langone. Kourtis says currently one in five children treated for the disease relapses within a decade. Attempts at blocking NOTCH1 directly have failed, he notes, because of adverse effects on healthy cells connected to the pathway.

The team genetically blocked HSF1 in mice induced (through increased NOTCH1 activity) to develop T-ALL, killing all cancer cells but not the mice. This evidence showed that HSF1 was essential to the survival of T-ALL cancer cells. The study also found that no adverse effects resulted and healthy blood cell production was not interrupted when HSF1 was removed from mouse bone marrow stem cells.

Further laboratory experiments in T-ALL mice and human cells showed that silencing the gene behind production of HSP90 efficiently killed leukemia cells, especially those with the highest NOTCH1 and HSP90 activity, the report says.

Dr. Aifantis says his team next plans to evaluate the effects of another eight proteins produced by genes active in the HSF1 pathway to see if any show promising anticancer activity in T-ALL. The team also hopes to launch clinical trials using HSP90 inhibitors against T-ALL.